ABSTRACT
Establishment of a cell-mediated gene transfer system has potential as a new breeding technique for commercially valuable fishes. As an important step toward developing an inter-species chimera, cells from blastula-stage embryos of rosy barb (Puntius conchonius) were transplanted into zebrafish (Danio rerio) blastula-stage embryos to observe the development of the recipient. From the total of 473 transplants obtained only a fraction of 13 chimeras appeared perfectly normal after one month. Over two in normal 13 chimeras showed some characters from the donor cells with scarce pigmentation. This is the first successful inter-species study on zebrafish by using blastula cell transplants from rosy barb both belonging to the same family cyprinidae.
Subject(s)
Animals , Base Sequence , Breeding , DNA Primers/genetics , Gene Transfer Techniques , Phenotype , Pigmentation , Stem Cell TransplantationABSTRACT
1. The level of lactic acid was found to be 25 mg percent in 95 percent of 186 normal Indians. There was no difference due to sex and age. 2. Level of lactic acid was estimated in blood of normal persons and diabetics Type II patients to observe the effects of food and glucose. There was no change except the level of lactic acid was in higher but in normal range. 3. Hyperglycemia of over 300 mg raised the blood lactic acid in 25 percent of patients. 4. Lactic acid was not affected by hypercholesteremia but was raised in 60 percent of cases with raised blood urea. 5. Lactic acid was found to remain within normal limits in 48 type II diabetics treated with phenformin dose varying from 50 mg to 225 mg per day. The duration of treatment varied from one year to seven years.
Subject(s)
Diabetes Mellitus, Type 2/blood , Diet , Female , Humans , Hypercholesterolemia/blood , Hyperglycemia/blood , India , Lactic Acid/analysis , Male , Phenformin/administration & dosage , Reference Values , Urea/bloodABSTRACT
1. The level of lactic acid was found to be between 5mg 25mg percent in 95 percent in 186 normal Indias. There was no difference due to sex and age. 2. Level of lactic acid was estimated in blood of normal persons and diabetes type II patients to observe the effects of food and glucose. The effect of food and and glucose was to take the levels of lactic acid to higher level than normal in about 25 percent of persons examined. 3. Hyperglycemia of over 300 mg raised the blood lactic acid in 25 percent of patients. 4. Lactic acid was not affected by hypercholereteremia but was raised in 60 percent of cases with high blood urea.